Figure 4.

Time-lapse imaging and plot of SF-CNP from Day 0 to Day 5 using Nikon’s BioStation IM. Only a few intermediate images of SF-CNP on Days 0, 3, and 5 at 40X magnification are shown. The white arrows mark some large SF-CNP on the same spot throughout the experiment. Note the small SF-CNP within the square blocks showing an increase in size and number over time. A graph of SF-CNP count against time in hours shows a linear increase in the SF-CNP number. The images and graph together imply an increase in size and number of SF-CNP over a period of 5 days. Bars: (A), (B), and (C) = 15 μm.

Abbreviation: SF-CNP, serum-free calcifying nanoparticles.