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. 2008 Sep 15;3(9):e3213. doi: 10.1371/journal.pone.0003213

Figure 3. In vitro binding of Adr1DBD and Ser98 mutants.

Figure 3

A. Electrophoretic mobility shift assays (EMSA) were performed with increasing amounts of Adr1 DBD (amino acids 17–160) and its Ser98Ala (S98A) and Ser98Asp (S98D) variants, against a fluorescently labeled double-stranded oligonucleotide containing the Adr1 binding site (UAS1) from the ADH2 promoter. Blue line, wildtype; red line S98A; green line S98D. Error bars represent the standard deviation of the mean measured in duplicate experiments. B. EMSA gel showing mobility shifts of the ADH2 promoter probe with wildtype Adr1 DBD or the S98D mutant.