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Western blots using antibody against phosphorylated serine 98 of Adr1 (α-pS98) or total Adr1 (α-Adr1) were performed on 99–125 µg of cell extract per lane from BY4741 wildtype or strains with the indicated kinase gene deletion. Each strain carried the high-copy Adr1 plasmid pNKA1-U.
