Table 1. Yeast strains.
strain | genotype | source |
BY4741 and kinase deletions | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 and relevant gene knockout with kanMX | [43] |
CKY13 | MATa ade2-1 can1-100 ura3-1 leu2-3,112 trp1-1 his3-11,115 Δadr1::kanMX | this work |
VBY20 | MATα adh3 ura3 his3 leu2::(pRS315-ADR1)X3 ADH2::YIpADH2/lacZ(TRP1) ADH2::YIpADH2/GFP(URA3) ADR1-3HA::kanmx | [9] |
NK85 | VBY20 ADR1-3HA::kanmx | this work |
NK87 | NK85 Δsnf1::kanmx | this work |
NK135 | NK85 Δpho85::NAT1 | this work |
NK136 | NK85 Δpho80::NAT1 | this work |
NK139 | NK85 Δpcl10::NAT | this work |
TYY201 | MATa ade2 can1-100 his3-11,115 leu2-3,112 trp1-1 ura3-1 | [32] |
TYY497 | MATa ade2 can1-100 his3-11,115 leu2-3,112 trp1-1 ura3-1 Δadr1::LEU2 ADH2::YIpADH2/lacZ(trp1::HIS3) | [32] |