Table 2. Methods for obtaining expression patterns, for the specified numbers of WT and mutant embryos.

Method	Genes
	hb WT bcd WT	hb 14F bcd WT	hb WT bcd K57R	hb WT bcd E1/+	lacZ	hb WT RNA hb WT prot.
FISHa	–	–	–	–	9	13
Immunofluorescenceb	50	39	23	7	d	13
Enzymatic stainingc	–	–	–	–	21	d

^aWhole mount fluorescent in situ hybridization.

^bFluorescence immunostaining.

^cWhole mount in situ hybridization with digoxygenin-labeled RNA.

^dAll lacZ embryos were co-stained for Bcd and Hb proteins.