Table 1.
peptide | Kd (μM) | ΔG (kcal/mol) | ΔH (kcal/mol) | TΔS (kcal/mol) | |
---|---|---|---|---|---|
1 | b QTSV | — | — | — | — |
2 | KQTSV | 18 ± 3 | −6.7 ± 0.1 | −4.7 ± 0.5 | 2.0 ± 0.6 |
3 | YKQTSV | 0.76 ± 0.04 | −8.4 ± 0.0 | −6.3 ± 0.2 | 2.1 ± 0.1 |
4 | NYKQTSV | 2.1 ± 0.1 | −7.8 ± 0.1 | −7.1 ± 0.1 | 0.7 ± 0.1 |
5 | KNYKQTSV | 0.47 ± 0.01 | −8.6 ± 0.1 | −6.6 ± 0.1 | 2.0 ± 0.1 |
6 | TKNYKQTSV | 0.80 ± 0.05 | −8.3 ± 0.1 | −6.6 ± 0.1 | 1.7 ± 0.1 |
7 | DTKNYKQTSV | 2.0 ± 0.1 | −7.8 ± 0.1 | −6.4 ± 0.1 | 1.4 ± 0.1 |
Values are the arithmetic mean of at least two independent experiments. All c values ranged between 5 and 162. Stoichiometric (n) values ranged from 0.90 to 1.06.
Insufficient heat detected to determine binding parameters.