Table 5.

ITC-determined thermodynamic binding parameters for PDZ3 and KKETEV-derived peptides. ^a The underlined residues indicate changes with respect to the parent peptide, KKETEV.

	peptide	Kd (μM)	ΔG (kcal/mol)	ΔH (kcal/mol)	TΔS (kcal/mol)
1	KETEV	18 ± 1	−6.5 ± 0.1	−4.3 ± 0.3	2.2 ± 0.3
2	KKETEV	1.9 ± 0.1	−7.8 ± 0.1	−6.2 ± 0.1	1.6 ± 0.1
3	KKKETEV	1.3 ± 0.1	−8.0 ± 0.1	−5.9 ± 0.3	2.1 ± 0.2
4	KKETEA	91 ± 2	−5.5 ± 0.1	−4.6 ± 0.2	0.9 ± 0.2
5	KKETEL	7.9 ± 1.3	−7.0 ± 0.1	−4.1 ± 0.3	2.9 ± 0.2
6	KKETEI	7.7 ± 1.2	−7.0 ± 0.1	−4.3 ± 0.2	2.7 ± 0.1
7	KKETEM	21 ± 2	−6.4 ± 0.1	−6.8 ±± 0.2	−0.4 ± 0.1
8	b KKETEF	57 ± 2	−5.8 ± 0.1	−4.4 ± 0.4	1.4 ± 0.4
9	KKETET	105 ± 6	−5.4 ± 0.1	−5.9 ± 0.2	−0.5 ± 0.2
10	KKESEV	6.6 ± 0.9	−7.1 ± 0.1	−4.8 ± 0.1	2.3 ± 0.2
11	KKECEV	72 ± 7	−5.7 ± 0.1	−1.7 ± 0.1	4.0 ± 0.2
12	KKESEL	33 ± 2	−6.1 ± 0.1	−4.0 ± 0.1	2.1 ± 0.1
13	KKESEI	24 ± 6	−6.3 ± 0.2	−5.0 ± 0.2	1.3 ± 0.4
14	b KKESEF	98 ± 16	−5.5 ± 0.1	−3.1 ± 0.1	2.4 ± 0.1
15	KKETGV	2.4 ± 0.0	−7.7 ± 0.1	−5.7 ± 0.2	2.0 ± 0.2
16	KKETAV	0.45 ± 0.10	−8.7± 0.1	−5.3 ± 0.4	3.4 ± 0.4
17	KKETVV	1.3 ± 0.2	−8.1 ± 0.1	−5.9 ± 0.1	2.2 ± 0.1
18	KKETLV	1.8 ± 0.3	−7.8 ± 0.1	−3.7 ± 0.4	4.1 ± 0.3
19	KKETPV	0.95 ± 0.15	−8.2 ± 0.1	−4.3 ± 0.1	3.9 ± 0.2
20	KKETWV	2.8 ± 0.4	−7.6 ± 0.1	−3.5 ± 0.2	4.1 ± 0.1
21	KKETDV	20 ± 2	−6.4 ± 0.1	−4.1 ± 0.3	2.3 ± 0.3
22	KKETKV	1.2 ± 0.0	−8.1 ± 0.1	−5.6 ± 0.6	2.5 ± 0.6
23	KKGTEV	80 ± 3	−5.6 ± 0.1	−2.7 ± 0.1	2.9 ± 0.1
24	KKATEV	21 ± 4	−6.4 ± 0.1	−2.4 ± 0.1	4.0 ± 0.2
25	KKQTEV	4.0 ± 0.0	−7.4 ± 0.1	−4.9 ± 0.3	2.5 ± 0.3
26	KKDTEV	85 ± 12	−5.6 ± 0.1	−3.9 ± 0.3	1.7 ± 0.2
27	KKKTEV	27 ± 4	−6.2 ± 0.1	−2.7 ± 0.3	3.5 ± 0.4
28	b KKGTGV	273 ± 30	−4.9 ± 0.1	−2.6 ± 0.3	2.3 ± 0.2
29	KKATAV	8.3 ± 1.5	−6.9 ± 0.1	−3.0 ± 0.1	3.9 ± 0.2
30	YKETEV	1.2 ± 0.1	−8.1 ± 0.1	−6.9 ± 0.1	1.2 ± 0.2

^aValues are the arithmetic mean of at least two independent experiments. All c values measured between 1 and 237. Stoichiometric (n) values ranged from 0.85 to 1.21.

^bITC was conducted as a competitive experiment.