Table 5.
peptide | Kd (μM) | ΔG (kcal/mol) | ΔH (kcal/mol) | TΔS (kcal/mol) | |
---|---|---|---|---|---|
1 | KETEV | 18 ± 1 | −6.5 ± 0.1 | −4.3 ± 0.3 | 2.2 ± 0.3 |
2 | KKETEV | 1.9 ± 0.1 | −7.8 ± 0.1 | −6.2 ± 0.1 | 1.6 ± 0.1 |
3 | KKKETEV | 1.3 ± 0.1 | −8.0 ± 0.1 | −5.9 ± 0.3 | 2.1 ± 0.2 |
4 | KKETEA | 91 ± 2 | −5.5 ± 0.1 | −4.6 ± 0.2 | 0.9 ± 0.2 |
5 | KKETEL | 7.9 ± 1.3 | −7.0 ± 0.1 | −4.1 ± 0.3 | 2.9 ± 0.2 |
6 | KKETEI | 7.7 ± 1.2 | −7.0 ± 0.1 | −4.3 ± 0.2 | 2.7 ± 0.1 |
7 | KKETEM | 21 ± 2 | −6.4 ± 0.1 | −6.8 ±± 0.2 | −0.4 ± 0.1 |
8 | b KKETEF | 57 ± 2 | −5.8 ± 0.1 | −4.4 ± 0.4 | 1.4 ± 0.4 |
9 | KKETET | 105 ± 6 | −5.4 ± 0.1 | −5.9 ± 0.2 | −0.5 ± 0.2 |
10 | KKESEV | 6.6 ± 0.9 | −7.1 ± 0.1 | −4.8 ± 0.1 | 2.3 ± 0.2 |
11 | KKECEV | 72 ± 7 | −5.7 ± 0.1 | −1.7 ± 0.1 | 4.0 ± 0.2 |
12 | KKESEL | 33 ± 2 | −6.1 ± 0.1 | −4.0 ± 0.1 | 2.1 ± 0.1 |
13 | KKESEI | 24 ± 6 | −6.3 ± 0.2 | −5.0 ± 0.2 | 1.3 ± 0.4 |
14 | b KKESEF | 98 ± 16 | −5.5 ± 0.1 | −3.1 ± 0.1 | 2.4 ± 0.1 |
15 | KKETGV | 2.4 ± 0.0 | −7.7 ± 0.1 | −5.7 ± 0.2 | 2.0 ± 0.2 |
16 | KKETAV | 0.45 ± 0.10 | −8.7± 0.1 | −5.3 ± 0.4 | 3.4 ± 0.4 |
17 | KKETVV | 1.3 ± 0.2 | −8.1 ± 0.1 | −5.9 ± 0.1 | 2.2 ± 0.1 |
18 | KKETLV | 1.8 ± 0.3 | −7.8 ± 0.1 | −3.7 ± 0.4 | 4.1 ± 0.3 |
19 | KKETPV | 0.95 ± 0.15 | −8.2 ± 0.1 | −4.3 ± 0.1 | 3.9 ± 0.2 |
20 | KKETWV | 2.8 ± 0.4 | −7.6 ± 0.1 | −3.5 ± 0.2 | 4.1 ± 0.1 |
21 | KKETDV | 20 ± 2 | −6.4 ± 0.1 | −4.1 ± 0.3 | 2.3 ± 0.3 |
22 | KKETKV | 1.2 ± 0.0 | −8.1 ± 0.1 | −5.6 ± 0.6 | 2.5 ± 0.6 |
23 | KKGTEV | 80 ± 3 | −5.6 ± 0.1 | −2.7 ± 0.1 | 2.9 ± 0.1 |
24 | KKATEV | 21 ± 4 | −6.4 ± 0.1 | −2.4 ± 0.1 | 4.0 ± 0.2 |
25 | KKQTEV | 4.0 ± 0.0 | −7.4 ± 0.1 | −4.9 ± 0.3 | 2.5 ± 0.3 |
26 | KKDTEV | 85 ± 12 | −5.6 ± 0.1 | −3.9 ± 0.3 | 1.7 ± 0.2 |
27 | KKKTEV | 27 ± 4 | −6.2 ± 0.1 | −2.7 ± 0.3 | 3.5 ± 0.4 |
28 | b KKGTGV | 273 ± 30 | −4.9 ± 0.1 | −2.6 ± 0.3 | 2.3 ± 0.2 |
29 | KKATAV | 8.3 ± 1.5 | −6.9 ± 0.1 | −3.0 ± 0.1 | 3.9 ± 0.2 |
30 | YKETEV | 1.2 ± 0.1 | −8.1 ± 0.1 | −6.9 ± 0.1 | 1.2 ± 0.2 |
Values are the arithmetic mean of at least two independent experiments. All c values measured between 1 and 237. Stoichiometric (n) values ranged from 0.85 to 1.21.
ITC was conducted as a competitive experiment.