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. Author manuscript; available in PMC: 2008 Sep 2.
Published in final edited form as: Biochemistry. 2007 Apr 4;46(17):4943–4950. doi: 10.1021/bi061738h

Figure 1. Summary of paramagnetic labeling strategies used in this study.

Figure 1

(a) The sequences of three DNA molecules that were synthesized and used in this study. “u” denotes deoxy-4-thiouracil and the red “T” denotes the 3′-end of phosphothioate. (b) The spin-labeling strategies using both modified base and phosphothiate are employed as depicted here. Both deoxy-4-thiouracil and the 3′-end of phosphothioate reacts with 3-(2-Iodoacetamido)-proxyl and thus allowed the covalent attachment of the spin-label to one end of a DNA molecule.