FIGURE 7.
Western analysis of inhibition of PR protein expression by agLNAs with different predicted thermal stabilities. Inhibition of PR by LNA PR10 (predicted Tm of 76°C), LNA PR3 (predicted Tm of 83°C), and LNA PR11 (predicted Tm of 91°C) shown at 25 nM (A) and at 50 nM (B). Percent inhibition of PR–B is relative to the average of noncomplementary LNAs LNAscr1 and LNAscr2. All agLNAs are complementary to −9 to +10 relative to the transcriptional start site of PRB. ni: no significant (<30%) inhibition.