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. 2008 Jul 22;99(3):491–501. doi: 10.1038/sj.bjc.6604486

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Copyright © 2008 Cancer Research UK

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Detection of claudin-4 in cell lines using western blot analysis. Cell lysates from the metastatic prostate cell lines (ALVA41, DU145, LNCaP and PC3), a normal prostate cell line (RWPE1) and a negative-control cell line (HeLa) were run on a 15% SDS-PAGE under reducing conditions and probed with an anti-claudin-4 antibody. High levels of claudin-4 were visible in the prostate tumour cell lines with a low level detected in RWPE1. No claudin-4 was detected in the negative control cell line, HeLa. Detection of the proliferating cell nuclear antigen (PCNA) served as a loading control.