Table 3. Immunohistochemical evaluation of AMACR, PSMA and claudin-4 expression in prostate tissue sections.
|
Percentage and intensity of cells staining
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| <25% | 25–50% | >50% | <25% | 25–50% | >50% | <25% | 25–50% | >50% | |
| Normal prostate sections | AMACR (n=15) | PSMA (n=14) | Claudin-4 (n=29) | ||||||
| Nil | 12 | 1 | 3 | ||||||
| Low | 3 | 3 | 6 | 2 | 3 | 12 | 4 | ||
| Moderate | 1 | 1 | 5 | 2 | |||||
| Strong | |||||||||
| Intense | |||||||||
| BPH | AMACR (n=14) | PSMA (n=13) | Claudin-4 (n=19) | ||||||
| Nil | 12 | 2 | 1 | ||||||
| Low | 1 | 1 | 1 | 2 | 4 | 1 | 3 | ||
| Moderate | 1 | 2 | 1 | 1 | 6 | 5 | |||
| Strong | 2 | ||||||||
| Intense | |||||||||
| HG-PIN (with no foci of PCa) | AMACR (n=11) | PSMA (n=11) | Claudin-4 (n=18) | ||||||
| Nil | 5 | 4 | |||||||
| Low | 3 | 1 | 6 | 1 | 2 | ||||
| Moderate | 1 | 1 | 12 | ||||||
| Strong | 1 | 1 | 1 | 1 | |||||
| Intense | |||||||||
| PCa | AMACR (n=21) | PSMA (n=21) | Claudin-4 (n=25) | ||||||
| Nil | 3 | ||||||||
| Low | 3 | 2 | 1 | 2 | 2 | 1 | 1 | ||
| Moderate | 1 | 2 | 2 | 3 | 5 | 3 | 9 | 4 | |
| Strong | 1 | 6 | 1 | 1 | 6 | 3 | 2 | 1 | |
| Intense | 1 | 1 | |||||||
| HG-PIN (with foci of PCa) | AMACR (n=19) | PSMA (n=18) | Claudin-4 (n=21) | ||||||
| Nil | 7 | ||||||||
| Low | 4 | 2 | 4 | 2 | 1 | ||||
| Moderate | 2 | 2 | 3 | 5 | 2 | 4 | |||
| Strong | 1 | 1 | 3 | 1 | 8 | 6 | |||
| Intense | |||||||||
| Benign glands (adjacent to PCa) | AMACR (n=21) | PSMA (n=21) | Claudin-4 (n=25) | ||||||
| Nil | 20 | 3 | |||||||
| Low | 1 | 5 | 3 | 3 | |||||
| Moderate | 2 | 4 | 1 | 7 | 7 | ||||
| Strong | 2 | 6 | 3 | ||||||
| Intense | |||||||||
| Metastatic sites | AMACR (n=18) | PSMA (n=16) | Claudin-4 (n=45) | ||||||
| Nil | 11 | 3 | |||||||
| Low | 5 | 4 | 1 | 3 | 1 | 9 | |||
| Moderate | 6 | 2 | 3 | 1 | 4 | 2 | 6 | 5 | |
| Strong | 6 | 4 | 2 | 1 | 3 | 13 | |||
| Intense | 1 | 3 | |||||||
Abbreviations: AMACR=α-methylacyl-CoA racemase; BPH=benign prostatic hyperplasia; HG-PIN=high-grade prostatic intraepithelial neoplasia; PCa=prostate cancer; PSMA=prostate-specific membrane antigen.
Immunohistochemical staining for AMACR, PSMA and claudin-4 was performed on a number (n) of prostate sections. The percentage and the intensity of cells staining within each of the sections were determined by a uropathologist.