Figure 5.

Immunoelectron microscopy of PLCγ1. Resting (A) and antigen-activated (B-D) (1 μg/ml DNP-BSA, 10 min) RBL-2H3 cells were embedded in LR White and thin sections labeled sequentially with anti-PLCγ1 and 15 nm Protein A-gold particles. Membrane-associated gold particles marking the location of PLCγ1 are circled in all panels to emphasize the concentration of membrane-associated PLCγ1 in membrane projections. Panels A and B show the presence of additional gold particles in the cell interior, including the nucleus. Micrographs are representative of results in three different experiments. Bar = 0.5 μm.