Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Covalent reactivity of A.17 and A.5 and their mutants. Concentrations of scFvs were normalized as confirmed by comparable staining in anti-c-Myc blot (lower panel). (B) Binding efficiency of clones selected after the third round, including reactive clones A.5, A.17 and their non-reactive mutants A.5Y32F and A.17Y36F. Non-selected clone (N) and anti-thyroglobulin scFv (anti-Thyr) were included as negative controls. Binding was determined as described in Methods.
