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. Author manuscript; available in PMC: 2008 Dec 26.
Published in final edited form as: J Am Chem Soc. 2007 Nov 29;129(51):16175–16182. doi: 10.1021/ja076528m

Figure 3.

Figure 3

(A) Covalent reactivity of A.17 and A.5 and their mutants. Concentrations of scFvs were normalized as confirmed by comparable staining in anti-c-Myc blot (lower panel). (B) Binding efficiency of clones selected after the third round, including reactive clones A.5, A.17 and their non-reactive mutants A.5Y32F and A.17Y36F. Non-selected clone (N) and anti-thyroglobulin scFv (anti-Thyr) were included as negative controls. Binding was determined as described in Methods.