(A) Diffusion coefficient distributions from Monte Carlo simulations of randomly diffusing particles on a lattice with varying amounts of sticky defects. Under defect-free conditions, the parameters are set so that the average diffusion coefficient is 1.0 μm2/s (green). With 10% surface coverage of sticky defects, the average diffusion coefficient is reduced to 0.52 μm2/s (red) and to 0.35 μm2/s (blue) at 20% surface coverage of sticky defects. (B) Example MSD vs. time interval plots of defect-free diffusion (green) and 20% sticky defect coverage (blue). In both cases, linear relationships are observed, demonstrating that if such defects exist, they cannot be detected as anomalous diffusion with the method of analysis used in our experiments.