(A) Virions with capsid proteins labeled in red, and RNA in green. In the overlay (merged) image, dual-labeled virions appear yellow or orange. (B) Release of RNA between 10 and 60 minutes post-infection. At 10 minutes, yellow dual-labeled virions are concentrated at the cell periphery; by 60 minutes, most virions are red, indicating loss of RNA. (C) Kinetics of RNA release. By approximately 25 minutes, half of the dual labeled virions have lost RNA. RNA release is not seen when virions are treated with an uncoating inhibitor, R78206. (Figure adapted from reference 16).