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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Trends Microbiol. 2008 Jan 10;16(2):44–47. doi: 10.1016/j.tim.2007.12.004

Figure 2. Poliovirus entry in brain microvascular endothelial cells.

Figure 2

(A) Virus enters the cell and moves to a perinuclear compartment. At 0 hrs, virus stained with anti-VP1 antibody (green) is seen at the cell surface most evident in x-z images. Poliovirus receptor (PVR, red) is also at the surface, largely concentrated at intracellular contacts. Cell nuclei are stained with DAPI (blue). By 2.5 hours post-infection, both virus and PVR have moved from the surface to a perinuclear compartment. (B) SHP-2 is required for virus entry. In control cells, virus (VP1, green) is seen within the cell, where it colocalizes with caveolin (CAV, red). In cells treated with an inhibitor of SHP-2 phosphatase, virus remains at the cell surface, largely at intercellular contacts. (Figure adapted from reference 17).