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Characterization of the DNA-binding activity of the wild-type MuB fusion protein. (A) Gel shift assay performed with 0.1 nM DNA fragment, FPWT, and presence or absence of ADP as indicated. The 80-bp fragment contained a single copy of the Arc operator sequence. (B) Same as in A except that the DNA fragment lacked the Arc operator sequence. Binding experiments in both A and B were repeated multiple times, and the data presented are representative curves.
