Fig. 4.
Kinetics of transposition reactions supported by MuB variants. (A) Agarose gel of products generated in the presence of MuB or FPWT in the presence of either ATP or ADP. Reactions were incubated for 2 h and contained Arc operator target plasmid (pCS13). DNA species are labeled as in Fig. 3. (B) Agarose gel of products generated in reactions containing FPWT, ATP, and the Arc operator target. Reactions were incubated for the length of time indicated. (C) Same as in B, although the MuB variant and target DNA were altered as indicated. (D) Graphical representation of the data from C.