Fig. 2.

Structural analysis of D96 and S93 particles. (A) Native PAGGE (8–25%) of D96 (lane 1) and S93 (lane 2). An 8–25% SDS/PAGGE analysis of cross-linked D96 (lane 6) and S93 (lane 7) is also shown. The particles were cross-linked at a 1:100 molar ratio of apoA-I: BS³ at 1 mg/ml apoA-I for PAGGE analysis to avoid partial cross-linking and to detect the highest MW bands. Lipid-free apoA-I (lane 4) and lipid-free apoA-I cross-linked under the same conditions (lane 5) is shown for comparison. All gels were stained with Coomassie Blue. (B and C) Negative stain EM images of D96 and S93 particles, respectively. (Scale bars: 50 nm.) (D) Experimental cross-links compatible with 5/5 molecular registry of the double-belt model are shown as for Fig. 1. Cross-linker color coding is the same as in Fig. 1.