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. 2008 Aug 22;105(34):12182–12187. doi: 10.1073/pnas.0804700105

Fig. 4.

Fig. 4.

pes-ARKO-TRAMP mice develop more aggressive and invasive tumors than Wt-TRAMP littermates. (A) PLN metastases are significantly larger in 24-wk-old pes-ARKO-TRAMP mice compared to Wt-TRAMP mice. (B) Weights of PLN metastases isolated from 24-wk-old pes-ARKO-TRAMP were greater than those of Wt-TRAMP mice (n = 7 mice in each group). (C) The number of liver tumor foci was increased in pes-ARKO-TRAMP mice compared to Wt-TRAMP mice (n = 6 mice in each group). (D) Western blot analysis of AR protein in PLN tumor, from 24-wk-old Wt-TRAMP or pes-ARKO-TRAMP mice. (E) Higher invasiveness of primary cultured PLN tumor cells from pes-ARKO-TRAMP mice as compared to those from Wt-TRAMP mice using Boyden chamber invasion assays (Upper). Adding functional AR by retrovirus infection into primary-cultured pes-ARKO-TRAMP tumor cells results in suppression of invasion (Lower). The purity and originality of primary cultured PLN tumor cells was confirmed by the expression of pan-CK epithelial cell marker (data not shown). Data were presented as mean ± SD (n = 5); *, P < 0.05; **, P < 0.01. (F) survival rate was decreased in pes-ARKO-TRAMP (C57BL/6 × TRAMP-FVB, n = 10) as compared with Wt-TRAMP (C57BL/6 x TRAMP-FVB, n = 16).