Figure 11.

Chymotrypsin resistance of soluble Grl1p. Isolated granules were incubated at 95°C for 30 min, and then sedimented at 150,000 × g for 2 h. Aliquots of the supernatant, containing ∼7 μg of protein, were incubated with or without 1 mM calcium, followed by 10 mM EGTA, as indicated. Chymotrypsin was then added at the indicated concentrations. Samples were visualized by antibody blotting after SDS-PAGE. Grl1p is marked by ∗.