Table 2.

Binding of Bcl-2 protein to peptides from ASPP2_Ank-SH3: Fluorescence spectroscopy studies

Parent protein	Peptide no. in array	Residues	Sequence	Kd, nM
ASPP2	14	931–942	LDSSLEGEFDLV	142 ± 38
	15	945–961	IIYEVDDPSLPNDEGIT	210 ± 36
	23	988–1002	ADSDGWTPLHCAASC	158 ± 17
	24	993–1004	*WTPLHCAASCNN	476 ± 40
	32	1039–1053	GYTQCSQFLYGVQEK	No binding
	37	1065–1082	LWDYEPQNDDELPMKEGD	96 ± 17

*Trp was added at the N terminus of some peptides for UV spectroscopy.

^†ASPP2 978–988 was not tested because we were unable to label it with fluorescein.