Table 1.
Dependent measures | Experimental condition |
||||||
---|---|---|---|---|---|---|---|
Diestrus | Behavior at estrus | Biosynthesis inhibitor to VTA | Metabolism inhibitor to VTA | Biosynthesis and metabolism inhibitor to VTA | OVX+SC E2+ vehicle to VTA | OVX+SC E2+3α,5α-THP to VTA | |
Central entries# | 6±1 | 17±6* | 0±0# | 2±1# | 3±1# | 13±12 | 98±8** |
Open arm time (s) | 28±4 | 110±12* | 2±2# | 9±6# | 8±8# | 21±12 | 34±5 |
Proximity to male (s) | 84±13 | 119±11 | 16±2# | 83±12# | 20±4# | 40±26 | 68±12** |
Social interaction (s) | 55±7 | 92±11* | 19±3# | 27±7# | 28±4# | 30±4 | 89±1** |
Lordosis quotient (%) | 10±2 | 94±2* | 46±9# | 50±3# | 51±9# | 25±6 | 75±11** |
Midbrain 3α,5α-THP (nmol/g) | 6±3 | 25±3* | 13±1# | 12±1# | 11±1# | 13±6 | 25±6** |
All groups represent three to 12 observations per group.
Indicates that analyses of variance (ANOVAs) revealed these parameters were significantly different for rats in behavioral estrous and diestrous.
Represents that ANOVAs show that rats in behavioral estrus that were infused with the biosynthesis and/or metabolism inhibitors (digitoxin and finasteride, respectively) to the VTA are significantly different from rats in behavioral estrous infused with vehicle.
ANOVAs show that ovx, E2-primed rats with infusions of 3α,5α-THP to VTA are different from those that receive vehicle to the VTA.