Table 1.
Yeast strains and plasmids
| Genotype | Source | |
|---|---|---|
| Yeast strains | ||
| YW5-1B | MATa leu2-3, 112 trp1 ura3-52 | Tsukada and Ohsumi (1993) |
| YIT701 | MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2 | This study |
| YIT702 | MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2 | This study |
| [pAPG7myc-314, pAPG12HA-316] | ||
| YIT703 | MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2 | This study |
| [pAPG7myc-324, pAPG12HA-426] | ||
| YIT704 | MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2 | This study |
| [pYO324, pAPG12HA-426] | ||
| YIT7G333A | MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2 | This study |
| [pAPG7G333Amyc-314, pAPG12HA-316] | ||
| YIT7C507A | MATa leu2-3, 112 trp1 ura3-52 apg7Δ::LEU2 | This study |
| [pAPG7C507 Amyc-314, pAPG12HA-316] | ||
| YTS2 | MATa ura3-52 lys2-801 ade2-101 trp1 his3 | This study |
| leu2 pho8::pho8Δ60 apg7::HIS3 | ||
| PJ69-4A | MATa trp1-901 leu2-3, 112 ura3-52 his3-200 | James et al. (1996) |
| gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 | ||
| met2::GAL7-lacZ | ||
| Plasmids | ||
| pRS314 | CEN6 TRP1 | Sikorski and Hieter (1989) |
| pRS316 | CEN6 URA3 | Sikorski and Hieter (1989) |
| pYO324 | 2μ TRP1 | Homma et al. (1998) |
| pAPG7myc-314 | CEN6 TRP1 APG7-3 × cmyc epitope | This study |
| pAPG7myc-324 | 2μ TRP1 APG7-3 × cmyc epitope | This study |
| pAPG7G333Amyc-314 | CEN6 TRP1 APG7G333A-3 × cmyc epitope | This study |
| pAPG7C507Amyc-314 | CEN6 TRP1 APG7C507A-3 × cmyc epitope | This study |
| pAPG12HA-316 | CEN6 URA3 3 × HA epitope-APG12 | Mizushima et al. (1998) |
| pAPG12HA-426 | 2μ URA3 3 × HA epitope-APG12 | Mizushima et al. (1998) |
| pGAD-C1 | 2μ LEU2 PADH1::GAL4 AD | James et al. (1996) |
| pGAD-APG12 | 2μ LEU2 PADH1::GAL4 AD-APG12 | This study |
| pGBD-C1 | 2μ TRP1 PADH1::GAL4 BD | James et al. (1996) |
| pGBD-APG7 | 2μ TRP1 PADH1::GAL4 BD-APG7-3 × cmyc epitope | This study |
| pGBD-APG7G333A | 2μ TRP1 PADH1::GAL4 BD-APG7G333A-3 × cmyc epitope | This study |
| pGBD-APG7C507A | 2μ TRP1 PADH1::GAL4 BD-APG7C507A-3 × cmyc epitope | This study |