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. 2008 May 9;4(5):e1000063. doi: 10.1371/journal.ppat.1000063

Figure 8. vMIA suppression of HtrA2/Omi-dependent cmvPCD.

Figure 8

(A) Average number of live cells (±range) in cultures at 72 h post transfection of Towne-BAC DNA (500 ng) with 12 ng of HtrA2/Omi expression plasmid or control vector or post transfection of ΔUL37x1 DNA (500 ng) with 12 ng of HtrA2/Omi expression plasmid in the absence or presence of 15 ng vMIA-encoding plasmid or 12 ng of control vector in the absence or presence of 15 ng vMIA-encoding plasmid. (B) Percentage of dead cells at 96 and 120 h postinfection (MOI of 0.0001) with ΔUL37x1 in HF, vMIAmut-HF, or vMIA-HF cultures. 400 cells/foci were evaluated for each condition. (C) Immunoblot detection of native vMIA (vMIA-nat.), vMIA-myc, and vMIAmut-myc in cell lysates collected at various h postinfection (MOI of 3) with ΔUL37x1 or Towne-BAC. Top, α-myc antibody; bottom, α-vMIA antibody. (D) Percentage of dead cells at 72, 96, 120, and 148 h postinfection (MOI of 0.0001) with ΔUL37x1, Towne-BAC, ΔUL37x1R1, or ΔUL37x1R2. 100 cells/foci were evaluated for each condition.