Table 2.
HT-29 and Caco-2 semiquantitative EGFR immunostaining evaluation (FS Cod)
| HT-29 | Caco-2 | |||
| Membrane | Cytoplasm | Membrane | Cytoplasm | |
| NT | Intermediate | Intermediate | Low | Low |
| E | Negative | Intermediate | Negative | Low |
| Cx5 | Intermediate | Intermediate | Low | Low |
| Cx5 + E | Negative | Intermediate | Negative | Low |
| Cx10 | Intermediate | Low | Negative | Low |
| Cx10 + E | Negative | Intermediate | Negative | Low |
| Gb | Intermediate | Intermediate | Low | Low |
| Gb + E | Negative | Intermediate | Negative | Low |
Membrane = Membrane immunostaining Histoscore; Cytoplasm = Cytoplasmic immunostaining Histoscore; FS Cod = Final Score coded according to cut-off values (see Material and Methods).
NT = untreated; E = Epidermal growth factor 10 nmol/L; Cx5 = cetuximab 5 nmol/L;
Cx10 = cetuximab 10 nmol/L; Gb = gefitinib 1 μmol/L; Cx5 + E = cetuximab 5 nmol/L + Epidermal growth factor 10 nmol/L; Cx10 + E = cetuximab 10 nmol/L + Epidermal growth factor 10 nmol/L;
Gb + E = gefitinib 1 μmol/L + Epidermal growth factor 10 nmol/L.