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. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Biochemistry. 2007 Apr 21;46(19):5697–5708. doi: 10.1021/bi602354t

FIGURE 4. SDS-PAGE analysis of density gradient ultracentrifugation fractions of plasma of apoA-I-/- mice expressing the WT or mutant forms of apoA-I or the control protein GFP.

FIGURE 4

Fractionation of plasma was performed as described in Experimental Procedures. The fractions that were obtained from the plasma of mice expressing the WT apoA-I (A) or the carboxy-terminal mutants apoA-I[Δ(185-243)] (B), apoA-I[Δ(220-243)] (C), apoA-I[Δ(232-243)] (D), apoA-I[E191A/H193A/K195A] (E) or the control protein GFP (F) were subjected to SDS-PAGE and the protein bands were visualized by staining with Coomassie Briliant Blue. On the right side of panels A-F is shown the CE/TC ratio from a pool of lipoprotein fractions that correspond to the HDL region (fractions 4-8). The fractions that were obtained from the plasma of mice expressing the apoA-I[Δ(185-243)] were further analyzed by SDS-PAGE and Western blotting using an anti-human apoA-I antibody (G) as described in Experimental Procedures. The densities of the fractions are indicated on the top of the figure. Panel H, Lipids and apoA-I concentrations from a pool of lipoprotein fractions that correspond to the HDL region (fractions 4-8) expressed as mg/dl. TG, triglycerides; PL, phospholipids; TC, total cholesterol; %, percentage composition relative to the sum of TG, PL and TC values. PL/apoA-I is expressed as molar ratio.