TABLE 1. Oligonucleotide sequence of primers used in PCR amplifications.

Name	Sequence	Location of sequence
185F	5′-CTC AAG GAG AAC TGAa GGC GCC AGA CTG-3′	nt 612-638 of apoA-I cDNAb (sense) (amino acids +181 to +189)
185R	5′-CAG TCT GGC GCC TCA GTT CTC CTT GAG-3′	nt 638-612 (antisense) (amino acids +189 to +181)c
M32S	5′-CTG GCC GCGd TAC GCC GCC GCG GCC ACC GAG-3′	nt 638-665 of apoA-I cDNA (sense) (amino acids +189 to +198)
M32A	5′-CTC GGT GGC CGC GGC GGC GTA CGC GGC CAG TCT-3′	nt 665-633 of apoA-I cDNA (antisense) (amino acids +198 to +188)
AINOT F	5′ CCT CCG CGG ACA GGC GGC CGCe CAG GG 3′	nt 886-911 of apoA-I genomic sequencef that contains a NotI site (sense), intron 3 of apoA-I gene
AISAL R	5′ A CAT GTC GAC CCC CTT TCA GGG CAC CTG GCC TTG 3′	ACAT + SalI site + nt 1917-1894 of apoA-I genomic sequence (antisense), at 3′ end of apoA-I gene

^athe stop codon is underlined

^bnucleotide number of the human apoA-I cDNA sequence (57), oligonucleotide position (+) relative to the translation initiation ATG codon

^camino acid position (+) refers to the mature plasma apoA-I sequence

^dmutagenized residues are marked in boldface type and are underlined

^ethe restriction enzyme recognition sites are marked in boldface type

^fnucleotide number of the human apoA-I genomic sequence (21), oligonucleotide position (+) relative to the translation initiation ATG codon