Fig. 8.

Evidence that myosin IIA is a functional motor for Naked2-associated vesicles. A, Naked2 antibody R44 immunoprecipitation (IP) products were resolved using SDS-PAGE and stained with Coomassie Blue. Bands were cut out, treated with modified trypsin, and identified by peptide mass fingerprinting using the MASCOT software (Matrix Science). Mass spectra were obtained as described under “Experimental Procedures.” Protein scores greater than 74 were significant (p < 0.05). The score for this band was 349, and the predicted molecular weight of the identified protein was consistent with the corresponding region of the gel as determined by the molecular weight markers. B, MDCK cells stably transfected with Naked2-EGFP were grown on coverslips for 2 h, and then either DMSO or blebbistatin was applied at a concentration of 5 μm for 16 h. Bar, 10 μm.