Skip to main content
. Author manuscript; available in PMC: 2008 Sep 3.
Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):192–199. doi: 10.1021/bi061706u

Figure 1.

Figure 1

Cleavage reactions and modified substrates for vTopo. (A) Reversible and suicide cleavage reactions. Strands with less than 6 nucleotides 3′ to the cleaved position spontaneously dissociate making the reaction irreversible. (B) Substrate series with truncated scissile strands and base substitutions at the +5, +4 and +3 guanine positions. Nucleotides that extend 3′ to the cleavage site on the scissile strand are given negative numbers and those that extend 5′ are given positive numbers.