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Suicide cleavage of 18/32mer and its N5 and ϕ4 analogues. (A) The increase in 2-aminopurine fluorescence of the 18/32mer and 18/32N5mer upon suicide DNA strand cleavage by vTopo is followed using stopped-flow fluorescence. (B) Initial rate of 18/32ϕ4 strand cleavage was measured using manual chemical quench methods with excess enzyme. The time dependence for formation of the covalent complex (upper band) provides the rate of suicide cleavage.
