Figure 1.

HvWRKY38 specifically represses GA induction of the Amy32b α-amylase promoter in aleurone cells. A, Schematic diagrams of the reporter and effector constructs used in the cobombardment experiment. B, The effector construct, UBI-HvWRKY38, was cobombarded into barley aleurone cells along with the reporter construct, Amy32b-GUS, and the internal control construct, UBI-Luciferase. The amount of reporter and internal control plasmid DNA was always constant, whereas that of the effector varied with respect to the reporter, as shown on the x axis. One hundred percent means that the same amount of effector and reporter DNA was used. GUS activity was normalized in every independent transformation relative to the same number of luciferase activity units (Lanahan et al., 1992). The lines indicate GUS activities ± se after 24 h of incubation of the bombarded aleurone cells with (+) or without (−) 1 μm GA. Data are means ± se of four replicates.