Figure 2.

Loss of HvWRKY38 activity leads to increased expression of Amy32b in the absence of GA. A, Schematic diagrams of gene constructs. Arrowheads indicate the orientations of the gene fragments. Numbers below the effector constructs represent the size (in base pairs) of every segment (not drawn to scale). B, The reporter construct, Amy32b-GUS, and the internal construct, UBI-Luciferase, were cobombarded into barley aleurone cells with the effector construct, UBI-Null or UBI-HvW38(RNAi), using the same molar ratio of effector and reporter constructs. GUS activity was normalized in every independent transformation relative to the luciferase activity. Lines indicate GUS activities ± se after 24, 48, or 72 h of incubation of the bombarded aleurone cells without GA. Data are means ± se of four replicates.