Figure 4.

Electrophoretic mobility shift assays of GST:HvGAMYB and GST:Hv-WRKY38 fusion proteins. A, The 61-bp probe (−171 to −111) and a 20- or 28-bp synthetic oligonucleotide competitor containing W-boxes or GARE were used in EMSA. Circles denote W-boxes, the diamond represents the pyrimidine box, and the rectangle represents GARE in the Amy32b promoter. The DNA probe was end labeled with [α-³²P]dATP. B, EMSA with recombinant GST:HvWRKY38 or GST:HvGAMYB proteins without (−) or with excess amounts of competitors (20- or 200-fold).