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Pairwise yeast two-hybrid interaction of MtSINA proteins, SINAT5, and SINAT5DN. The clones were fused to the GAL4 BD (horizontal) or GAL4 AD (vertical) and tested by a pairwise yeast two-hybrid analysis. Yeast colonies were spotted with equal density on −Trp/Leu/His medium in the presence of 10 mm 3-AT.
