Table III.
Sugar and starch in the lamina (excluding midrib) and petiole of the indicated lines
Transient carbohydrates in the lamina and petiole of the indicated lines were measured to assess the efficiency of transport out of the leaf and along the transport phloem. The first three adult leaves from a single plant were pooled for extraction, and carbohydrate levels were measured in three to five siblings. All values are nanomoles per milligram fresh weight, and starch is expressed as Glc equivalents.
Representative Line | Carbohydrate | Lamina | Petiole |
---|---|---|---|
AtSUC2 +/+ | Glc | 2.28 ± 0.95 | 0.51 ± 0.02 |
Fru | 0.80 ± 0.36 | 0.14 ± 0.03 | |
Suc | 1.72 ± 0.76 | 0.35 ± 0.07 | |
Starch | 59.5 ± 5.0 | 8.28 ± 3.68 | |
Total | 64.3 ± 4.4 | 9.28 ± 3.79 | |
SUC2p∷cSUC2 KD1039 | Glc | 1.40 ± 0.42 | 0.53 ± 0.15 |
Fru | 0.42 ± 0.12 | 0.12 ± 0.02 | |
Suc | 1.40 ± 0.40 | 0.35 ± 0.07 | |
Starch | 84.6 ± 18.2a | 12.02 ± 3.64 | |
Total | 87.8 ± 18.2a | 13.02 ± 3.60 | |
CmGAS1p∷cSUC2 KD1294 | Glc | 13.00 ± 2.7a | 1.36 ± 0.17a |
Fru | 3.78 ± 1.22a | 0.24 ± 0.03a | |
Suc | 5.76 ± 0.52a | 0.83 ± 0.17a | |
Starch | 639.5 ± 61.9a | 37.1 ± 7.0a | |
Total | 662.0 ± 65.8a | 39.5 ± 7.2a |
Student's t test, P < 0.05, relative to the wild type.