Skip to main content
. 2008 Sep;148(1):200–211. doi: 10.1104/pp.108.124776

Table IV.

Percentage of 14C-labeled photosynthate exuded from cut petioles and retained in the leaf at 20 h after photosynthetic labeling with 14CO2

Four mature adult leaves were excised and photosynthetically labeled with 14CO2. Phloem exudation into EDTA-containing solution was collected and analyzed by scintillation counting. Label remaining in leaves in soluble and insoluble fractions was also established, and label distribution is expressed as a percentage of the total. Experiments in the top and bottom portions of the table were carried out on different days and with different plants. Average and sd values are from four (top three entries) or three (bottom two entries) sibling plants.

Line Cumulative Percentage 14C Exudation
Soluble Residual Insoluble Residual
0 h 2 h 4 h 9 h 20 h
AtSUC2 +/+ 0.0 ± 0.0 20.8 ± 4.1 36.7 ± 6.5 56.4 ± 6.2 70.1 ± 6.2 19.4 ± 4.9 10.5 ± 2.2
KD1039 0.1 ± 0.2 16.6 ± 5.3 31.4 ± 8.7 52.8 ± 6.5 66.5 ± 5.7 21.4 ± 1.8 12.1 ± 5.3
KD1294 0.1 ± 0.0 19.3 ± 4.1 29.9 ± 6.2 45.7 ± 6.8 59.4 ± 5.0a 18.7 ± 4.1 21.9 ± 3.1a
AtSUC2 +/+ 0.0 ± 0.0 30.0 ± 2.9 47.2 ± 4.9 63.5 ± 3.7 75.3 ± 2.5 17.2 ± 0.8 7.5 ± 2.5
AtSUC2 −/− 0.0 ± 0.0 16.3 ± 5.8a 31.3 ± 10.2 52.2 ± 9.4 73.6 ± 3.8 18.1 ± 1.6 8.3 ± 2.7
a

Student's t test, P < 0.05, relative to AtSUC2 +/+, performed separately on different experiments.