Figure 2.

Fractionation of sea urchin axonemes. Axonemes (5 mg/ml) were sequentially incubated with 0.6 M NaCl (lane 1) for 15 min, at 40°C (lane 2) for 5 min, and with 0.5% Sarkosyl (lane 3) for 60 min. Lane 4 represents the final pellet of the extraction scheme. Aliquots (10 μl) of fractions collected at each step were subjected to 11% SDS-polyacrylamide gels and the proteins were stained with Coomassie blue (A) or immunoblotted with D-316 (B).