Figure 3.

Mono Q anion exchange chromatography of heat-extracted axonemal proteins. (A) The heat extract (2 mg of protein) was applied to a 1-ml Mono Q column previously equilibrated with 20 mM Tris-Cl (pH 8.0). The proteins were eluted at a flow rate of 0.5 ml/min using the indicated linear salt gradient, and 1-ml fractions were collected. The presence of p90 in the indicated fractions (upper panel) and in the starting extract (E) was monitored by immunoblotting with mAb D-316. (B) SDS-PAGE analysis of the immunoreactive fractions. Fractions 25 and 26 from the Mono Q column were pooled and an aliquot (10 μl) was analyzed by SDS-PAGE on a 11% gel.