Figure 4.

Immunoprecipitation of p90 and associated proteins from axonemal crude extracts. The crude heat extract (200 μg of protein, lane 1) was incubated in the absence (lane 2) or in the presence of the unrelated mAb D405–14 (lane 3), mAb D-316 (lanes 4 and 5), and the antitubulin D-66 produced in our laboratory (lane 6). The immune complexes were precipitated with protein G Sepharose beads, except for lane 5 where unconjugated Sepharose beads were used. The samples were subjected to SDS-PAGE and the resulting gel was stained with Coomassie blue. Lane 7 represents the protein composition of the Mono Q-active fraction pool.