Figure 5.

Partial amino acid sequencing of the active fraction from the Mono Q chromatography. (A) The immunoreactive fraction from the Mono Q column was subjected to SDS-PAGE and electroblotted to a PVDF membrane (same lane pool as in Figure 3B). Each separated band was digested with endoproteinase Lys-C and/or CNBr digestions and peptides were separated by high-pressure liquid chromatography were sequenced. The lysine residues in parentheses are deduced from the known specificity of endoproteinase Lys-C. Other residues in parentheses represent amino acids for which identification was carried out with a lower degree of confidence. (B) Search in databases indicated a significant similarity between two peptides from p90 with two peptides from radial spoke head proteins RSP4 and RSP6. Identities are indicated by vertical lines and similarities by dots.