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Expression of the cloned p63 as a polyhistidine-tagged protein. Purified native p63 and its associated proteins (Mono Q pool, N) and recombinant p63 (R) (2 μg each) were separated by SDS-PAGE and the resulting gel was stained with Coomassie blue (CBB) or immunoblotted with mAb D-316 (Blot). Molecular mass standards are indicated on the right.
