Figure 7.

Vector DNA accumulation is reduced at higher temperature. Leaves showing visible evidence of silencing from the CLCrV:ChlI vector were collected 47 dpi and used for DNA isolation. Lanes 1 to 3 show DNA from three different leaves from different plants grown at lower temperature (22°C/18°C, as in Fig. 5G) while lanes 4 to 6 show DNA isolated from plants grown at higher temperature (30°C/26°C; see Fig. 5C). A digoxigenin-labeled DNA probe corresponding to the coding region and part of AL1 was used. Ethidium bromide staining of the gel showed that equal amounts of genomic DNA were loaded in each lane (data not shown). OC, Open circular form of CLCrVA:ChlI; SC, supercoiled.