Figure 4.

Disulfide reductase activity of Trxs determined by the DTNB reduction assay. Different concentrations of Trxs were incubated in the presence of 1.6 μm MtNTRA, 0.2 mm NADPH, and 100 μm DTNB in 30 mm Tris-Cl, pH 8. DTNB reduction was followed at 412 nm.