Figure 2.

Influence of CDT on the proteome of Arabidopsis seeds. An equal amount (150 μg) of total soluble protein extracts was loaded on each gel. A, Silver-stained 2D gel of total soluble proteins from nondeteriorated seeds (0 d, control seeds). The indicated portions of the gel (a, b, c, and d) are reproduced in B. B, Enlarged windows (a–d) of 2D gels as shown in A for nondeteriorated seeds (left), 3-d deteriorated seeds (middle), and 7-d deteriorated seeds (right). The seven labeled protein spots (spots 7, 146, 212, 253, 254, 255, and 302) were identified by mass spectrometry and by comparison with Arabidopsis seed protein reference maps (Gallardo et al., 2001, 2002a; Rajjou et al., 2004, 2006a; Job et al., 2005; http://www.seed-proteome.com; Tables I and II; Supplemental Table S2). Protein spot quantitation was carried out as described in “Materials and Methods” from at least five gels for each seed sample.