Figure 3.

Dynamic evolution of protein spot abundance in Arabidopsis seeds submitted to CDT. The relative abundance of protein spots was calculated by dividing the normalized spot volumes in the deteriorated seeds (4 and 16 h, and 1, 2, 3, 5, and 7 d) by the corresponding normalized spot volumes in the nondeteriorated seeds (0 d). A, Time courses of relative abundance increase in deteriorated seeds during CDT for the two protein spots (spots 253 and 312). B, Time courses of relative abundance decrease in deteriorated seeds during CDT for the two protein spots (spots 146 and 311). Protein spot quantitation was carried out as described in “Materials and Methods” from at least five gels for each seed sample. Solid lines were obtained by nonlinear regression analysis using the following equations: relative abundance of spot = a − b.exp(−t/T_1/2) and relative abundance of spot = a + b.exp(−t/T_1/2), for increase and decrease in relative abundance, respectively, where a and b are constant parameters, T_1/2 is the time (days) needed to reach half variation in spot volume during the CDT, and t is the time of CDT (days). T_1/2 values are listed in Tables I, II, IV, and V.