Figure 7.

Comparison of de novo protein synthesis patterns during germination sensu stricto (1 d after imbibition) of deteriorated seeds. A, Protein profiles of de novo synthesized proteins in nondeteriorated seeds (0 d, control seeds). B, Protein profiles of de novo synthesized proteins in 3-d deteriorated seeds (3 d of CDT). C, Protein profiles of de novo synthesized proteins in 7-d deteriorated seeds (7 d of CDT). Radiolabeling of proteins was carried out by introducing [³⁵S]Met in the germination assays, as described in “Materials and Methods.” Soluble proteins were extracted after 1 d of imbibition and submitted to 2D gel electrophoresis, and the radiolabeled proteins were revealed as described in “Materials and Methods.” The 33 labeled protein spots were identified by mass spectrometry and by comparison with Arabidopsis seed protein reference maps (Gallardo et al., 2001, 2002a; Rajjou et al., 2004, 2006a; Job et al., 2005; http://www.seed-proteome.com; Table VI; Supplemental Table S2). a, 12S seed storage protein precursor; b, α-subunit of 12S seed storage protein; c, β-subunit of 12S seed storage protein. Several radiolabeled spots exhibiting contrasting specific accumulation in deteriorated seeds could not be identified because they had no match with proteins detected in Arabidopsis seed protein reference maps (http://www.seed-proteome.com).