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. 2008 Sep;148(1):620–641. doi: 10.1104/pp.108.123141

Table I.

Arabidopsis proteins whose abundance significantly increased in dry mature seeds according to controlled deterioration time

AGI, Arabidopsis Genome Initiative.

No.a Experimental Molecular Mass Experimental pI Arabidopsis Protein Name Theoretical Molecular Mass Theoretical pI AGI No. Coverage Relative Abundanceb T1/2
kD kD ratio 7 d:0 d d
7c 37.55 5.09 60S acidic ribosomal protein 34.37 5.08 At3g11250 24% 3.4 ± 0.1 2.2 ± 0.3
212d 42.57 5.18 Actin 2 41.21 5.43 At3g18780 33% 2.5 ± 0.4 5.2 ± 2.7
253d 40.29 5.84 Glyceraldehyde-3-P dehydrogenase, cytosolic 36.99 6.34 At3g04120 18% >16 >7
293d 96.69 5.61 Peptidase M1 family protein 103.40 6.09 At1g63770 4% >5.1 >7
302d 40.34 6.03 Glyceraldehyde-3-P dehydrogenase 36.90 7.21 At1g13440 30% 7.7 ± 1.4 6.1 ± 2.0
305d 68.25 5.70 Phosphoglucomutase 63.46 5.57 At1g70730 34% 2.2 ± 0.1 1.3 ± 0.4
308c 28.52 5.89 α-Cruciferin 12S (seed storage protein fragment) 50.56 6.53 At1g03880 10% 3.4 ± 0.5 4.8 ± 1.7
312c 14.21 3.82 NADP-dependent glyceraldehyde-3-P dehydrogenase (fragment) 53.04 6.61 At2g24270 8% 6.0 ± 0.3 1.8 ± 0.3
320c 57.36 5.82 Ribulose bisphosphate carboxylase large chain 53.47 6.25 AtCg00490 24% >2.7 >7
321c 57.36 5.84 Ribulose bisphosphate carboxylase large chain 53.47 6.25 AtCg00490 28% >2.8 >7
322c 35.21 5.20 60S acidic ribosomal protein 33.65 4.93 At2g40010 15% >4.4 >7
376d 57.35 4.92 Tubulin β-8 chain 50.59 4.46 At5g23860 9% 5.0 ± 0.2 1.5 ± 0.2
a

Protein numbering following Arabidopsis seed protein reference maps available at http://www.seed-proteome.com.

b

Data obtained from densitometric analysis of individual spots from proteins on 2D gels stained with silver nitrate (see Fig. 2A for an example of a 2D gel): normalized spot volume in the deteriorated seeds (7 d of CDT) divided by the normalized spot volume in the nondeteriorated control seeds (0 d of CDT), from five different gels and three independent extractions.

c

Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a; Rajjou et al., 2004, 2006a; Job et al., 2005).

d

Listed proteins correspond to proteins identified during this work; the peptide sequences determined are available in Supplemental Table S2.