Table IV.
Arabidopsis proteins whose abundance was significantly greater in deteriorated seeds than in control seeds during germination sensu stricto (1 d after imbibition)
| No.a | Experimental Molecular Mass | Experimental pI | Arabidopsis Protein Name | Theoretical Molecular Mass | Theoretical pI | AGI No. | Coverage | Relative Abundanceb | T1/2 |
|---|---|---|---|---|---|---|---|---|---|
| kD | kD | ratio 7 d:0 d | d | ||||||
| Energy metabolism | |||||||||
| 253c | 40.29 | 5.84 | Glyceraldehyde-3-P dehydrogenase, cytosolic | 36.99 | 6.34 | At3g04120 | 18% | >6.2 | >7 |
| 302c | 40.34 | 6.03 | Glyceraldehyde-3-P dehydrogenase | 36.90 | 7.21 | At1g13440 | 30% | >7.6 | >7 |
| 368d | 40.36 | 6.24 | Glyceraldehyde-3-P dehydrogenase | 36.90 | 7.21 | At1g13440 | 17% | >4.5 | >7 |
| 369d | 40.33 | 6.20 | Glyceraldehyde-3-P dehydrogenase | 36.90 | 7.21 | At1g13440 | 22% | >5.0 | >7 |
| 349d | 64.85 | 6.77 | Malate oxidoreductase | 64.24 | 6.68 | At2g19900 | 12% | >32.0 | >7 |
| 350d | 64.70 | 7.21 | Malate oxidoreductase | 64.24 | 6.68 | At2g19900 | 2% | >39.0 | >7 |
| Amino acid metabolism | |||||||||
| 359c | 56.90 | 5.57 | S-Adenosyl-l-homo-Cys hydrolase | 53.36 | 5.83 | At4g13940 or At3g23810 | 13% | >20.0 | >7 |
| 370c | 41.66 | 6.50 | Glutamate dehydrogenase | 44.51 | 6.02 | At5g18170 or At3g03910 | 13% | >2.5 | >7 |
| Seed storage proteins | |||||||||
| 354d | 42.61 | 6.60 | 12S seed storage protein (precursor) | 52.56 | 8.08 | At5g44120 | 25% | >9.2 | >7 |
| 177d | 45.47 | 6.07 | 12S seed storage protein (precursor) | 48.03 | 6.56 | At1g03880 | 17% | >3.0 | >7 |
| 151d | 46.11 | 5.38 | Cupin family protein | 49.66 | 5.45 | At1g03890 | >6.0 | >7 | |
| Hydrolase and protease | |||||||||
| 378c | 80.28 | 5.77 | Subtilisin-like Ser proteinase | 81.80 | 6.76 | At3g14067 | 19% | >3 | >7 |
| 360c | 64.96 | 5.75 | Glycosyl hydrolase family | 84.29 | 7.71 | At5g64570 | 14% | >3.0 | >7 |
| Other processes | |||||||||
| 139d | 40.07 | 5.56 | Reversibly glycosylated polypeptide | 40.7 | 5.61 | At3g02230 or At5g15650 | 7% | >2.5 | >7 |
| 377d | 38.42 | 6.58 | Potassium channel β-subunit | 36.52 | 7.49 | At1g04690 | 15% | >3.8 | >7 |
| 380d | 26.31 | 5.89 | Nucleoside diphosphate kinase II, chloroplast | 25.53 | 9.30 | At5g63310 | 12% | >3.8 | >7 |
Protein numbering following Arabidopsis seed protein reference maps available at http://www.seed-proteome.com.
Data obtained from densitometric analysis of individual spots from proteins on 2D gels stained with silver nitrate (see Fig. 2A for an example of a 2D gel): normalized spot volume in the deteriorated seeds (7 d of CDT) incubated for 1 d in water divided by the normalized spot volume in the nondeteriorated control seeds (0 d of CDT) incubated for 1 d in water, from five different gels and three independent extractions.
Listed proteins correspond to proteins identified during this work; the peptide sequences determined are available in Supplemental Table S2.
Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a; Rajjou et al., 2004, 2006a; Job et al., 2005).